Medium: Diatom

Acronym	ACUF Diatom
Description	Bacillariophycean Medium
Full description	Ca(NO3)2 . 4 H2O - 0.2 (stock solution [g/100 ml]) - 20 (nutrient solution [ml]) K2HPO4 - 0.1 (stock solution [g/100 ml]) - 10 (nutrient solution [ml]) MgSO4 . 7 H2O - 0.1 (stock solution [g/100 ml]) - 25 (nutrient solution [ml]) Na2CO3 - 0.1 (stock solution [g/100 ml]) - 20 (nutrient solution [ml]) Na2SiO3 . 9 H2O - 0.1 (stock solution [g/100 ml]) - 50 (nutrient solution [ml]) Fe-citrate - 0.1 (stock solution [g/100 ml]) - 10 (nutrient solution [ml]) citric acid - 0.1 (stock solution [g/100 ml]) - 10 (nutrient solution [ml]) soil extract * - 30 (nutrient solution [ml]) micronutrient solution ** - 5 (nutrient solution [ml]) de-ionized or distilled water 820 (nutrient solution [ml]) * Preparation of soil extract (as in medium 1): Fill a 6 litre flask one third with garden or leaf soil of medium, but not too great humus content which does not contain fertilizers or plant protective agents. Success of soil extract depends on selection of suitable soils. Those with high clay content are usually less satisfactory. Add de-ionized water until it stands 5 cm above the soil and sterilize by heating in a steamer for one hour twice in a 24 h interval. Separate the decanted extract from particles by centrifugation. Fill into small containers of stock solution each of a size appropriate to making a batch of media, autoclave for 20 min at 121°C and store in the refrigerator. ** Preparation of the micronutrient solution (as in medium 1) ZnSO4 . 7H2O - 0.1 (stock solution [g/100 ml]) - 1 ml (applied solution) MnSO4 . 4H2O - 0.1 (stock solution [g/100 ml]) - 2 ml (applied solution) H3BO3 - 0.2 (stock solution [g/100 ml]) - 5 ml (applied solution) Co(NO3)2 . 6H2O - 0.02 (stock solution [g/100 ml]) - 5 ml (applied solution) Na2 MoO4 .2H2O - 0.02 (stock solution [g/100 ml]) - 5 ml (applied solution) CuSO4 . 5H2O - 0.0005 (stock solution [g/100 ml]) - 1 ml (applied solution) de-ionized or distilled water 981 ml (applied solution) FeSO4 . 7H2O - 0.7 g (applied solution) EDTA (Titriplex III, Merck) 0.8 g (applied solution) Add vitamin B12 (5 x 10-6g/l) in sterile solution after cooling. a) Bacillariophycean Medium with Vitamins (= Diat Vit.mix.) Add following sterile vitamin-solutions after cooling: 1 ml of thiamine-solution (0.1 g/100 ml) 0.5 ml of biotin-solution (25 x 10-6 g/100 ml) 0.5 ml of vitamin B12-solution (10 x 10-6 g/100 ml)

Connected strains

Number of items per page

Number of elements: 1

Collection	Accession Number	Organism Type	Taxon Name
ACUF	100	Microalgae	Pinnularia obscura Krasske

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