Medium: Dunalialla acidophila

Acronym	ACUF Dunalialla acidophila
Description	Dunalialla acidophila Medium
Full description	MgSO4 . 7H2O (25.00 g), NH4NO3 (0.25 g), K2HPO4 (0.075 g), CaCl2 . 2H2O (0.012 g), peat extract * (5.0 ml ), micronutrient solution ** (5.0 ml), de-ionized or distilled water (990.0 ml). Adjust to pH 1.5 with 1N H2SO4. Add vitamin B12 (5x10-6g/l) in sterile solution after autoclaving. * Preparation of peat extract (as in medium 7): Fill a 6 litre flask one third with peat soil of medium, but not too great humus content which does not contain fertilizers or plant protective agents. Success of peat extract depends on selection of suitable soils. Add de-ionized water until it stands 5 cm above the soil and sterilize by heating in a steamer for one hour twice in a 24 h interval. Separate the decanted extract from particles by centrifugation. Fill into small containers of stock solution each of a size appropriate to making a batch of media, autoclave for 20 min at 121°C and store in the refrigerator. ** Preparation of the micronutrient solution (as in medium 1): ZnSO4 . 7H2O (1 ml), MnSO4 . 4H2O (2 ml), H3BO3 (5 ml), Co(NO3)2 . 6H2O (5 ml), Na2 MoO4 .2H2O (5 ml), CuSO4 . 5H2O (1 ml), de-ionized or distilled water (981 ml), FeSO4 . 7H2O (0.7 g), EDTA (Titriplex III, Merck) (0.8 g)

Connected strains

Number of items per page

Number of elements: 2

Collection	Accession Number	Organism Type	Taxon Name
ACUF	229	Microalgae	Dunaliella acidophila (Kalina) Massyuk
ACUF	435	Microalgae	Dunaliella acidophila (Kalina) Massjuk

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